August 22-23, 2018 Rome, Italy
Diego Fajardo1, P Cano-Barquilla, L Virto Ruiz, M J Perez de Miguelsanz, V Jimenez-Ortega, AI Esquifino and P Fernandez-Mateos2
1Universidad Complutense de Madrid, Spain
2Instituto de Investigacion Sanitaria Hospital Clínico San Carlos, Spain
Obesity is a multifactorial disease not well understood. This work was performed to analyze the effect of a high fat diet
administration on mesenteric fat lipolysis, triglycerides synthesis and circulating levels of insulin, glucose and triglycerides.
For this purpose, adult male rats were fed with a High Fat Diet (HFD), obesity group or standard rat chow, control group, for
three months. At this point, animals were sacrificed by decapitation to avoid stress effects. Immediately after, mesenteric fat
was aseptically dissected and together with the plasma from the trunk blood were kept to -80ºC until analyzed.
Relative gene expression was performed by RT-qPCR. As expected, plasma levels of insulin, triglycerides and blood glucose levels
were increased in HFD as compared to control group. Relative expression of Atgl gene was not modified either by HFD or
photoperiod phase. HFD decreased DAGT1, HSL and perilipin relative gene expression in dark phase of photoperiod, while
DAGT2 was decreased in the light phase.
However, HFD feeding markedly increased CGI-58 relative gene expression in the
dark phase, but no differences were found in the light phase of the photoperiod. The results suggest that obesity modify the
expression genes of the proteins involved in lipid metabolism in mesenteric fat.
Diego Fajardo is currently a Pharmacy student – Department of Biochemistry and Molecular Biology at Universidad Complutense de Madrid. His interest is in